HDL-Cholesterol

System reagent for the quantitative determination of HDL-Cholesterol concentrations in human serum and plasma on Beckman Coulter AU analyzers. The HDL-Cholesterol test is a two reagent homogenous system for the selective measurement of serum or plasma HDL-Cholesterol in the presence of other lipoproteins in solubilized and consumed by cholesterol oxidase, peroxidase, and DSBmT to generate a colorless and product. In phase two a unique detergent selectively solubilizes HDL-lipoproteins. The HDL cholesterol is released for reaction with cholesterol esterase, cholesterol oxidase and a chomogen system to yield a blue color complex which can be measured bichromatically at 600/700nm.