ACCESS TOTAL T3 2 X 50 DET
SKU
BKM-33830
The Access Total T3 assay is a paramagnetic particle, chemiluminescent immunoassay for the quantitative determination of triiodothyronine levels in human serum and plasma using the Access Immunoassay Systems.
| Assay Features | • Highly specific and sensitive performance • Reduced interference from heterophile antibodies • Compatibility with serum or heparinized plasma, which provides testing flexibility • Wide dynamic range, reducing the need for sample dilution |
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The Access Total T3 assay is a paramagnetic particle, chemiluminescent immunoassay for the quantitative determination of triiodothyronine levels in human serum and plasma using the Access Immunoassay Systems. The Access Total T3 assay is a competitive binding immunoenzymatic assay. Sample is added to a reaction vessel with a stripping agent to dissociate T3 from the binding proteins. T3 in the sample competes with the T3 analogue coupled to biotin for anti-T3 alkaline phosphatase conjugate. Of the resulting antigen: antibody complexes, the T3 analogue: antibody complexes are bound to the streptavidin coated solid phase. Separation in a magnetic field and washing removes the sample T3: antibody complexes and other materials not bound to the solid phase. Then, the chemiluminescent substrate Lumi-Phos* 530 is added to the vessel and light generated by the reaction is measured with luminometer. The light production is inversely proportional to the concentration of total T3 in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.